We are developing an interdisciplinary training environment composed of immunologists, engineers, computational biologists, and others that harnesses emerging techniques to answer fundamental questions of biological and clinical relevance in barrier tissue biology. We use a variety of techniques such as single-cell RNA-sequencing (scRNA-seq), organoid models, epigenetic profiling, flow cytometry, and microscopy in an effort to answer pressing questions surrounding human health and disease. The fundamental questions we try to answer through our work in the lab are: Which cellular compartments harbor memories of inflammation in tissue, and how might we develop effective mechanisms by which to promote or erase them? In short, where are health and disease stored in a tissue?